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abcf1 gene knockdown  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology abcf1 gene knockdown
    A) In bone marrow-derived macrophages (BMDMs), <t>ABCF1</t> was manipulated through either overexpression (Over Exp.) or treatment with specific siRNA. These interventions were carried out both in the presence and absence of lipopolysaccharide (LPS) stimulation (at a concentration of 100 ng/ml for 24 hours). The resulting heat map illustrates the fold change in various cytokines and chemokines detected in the cell culture supernatants. B) Similarly, in BMDMs, ABCF1 was modulated using Abcf1-specific siRNA, and these cells were exposed to LPS treatment. The heat map presented here showcases the fold change in various Mitogen-Activated Protein Kinase (MAPK) and Interferon-Stimulated Gene (ISG)-specific phosphoproteins and transcription factors detected in whole-cell lysates (WCL).
    Abcf1 Gene Knockdown, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abcf1 gene knockdown/product/Santa Cruz Biotechnology
    Average 92 stars, based on 4 article reviews
    abcf1 gene knockdown - by Bioz Stars, 2026-03
    92/100 stars

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    1) Product Images from "Phagocytosis in macrophages is regulated by the ATP-binding cassette family gene ABCF1"

    Article Title: Phagocytosis in macrophages is regulated by the ATP-binding cassette family gene ABCF1

    Journal: bioRxiv

    doi: 10.1101/2023.09.05.556419

    A) In bone marrow-derived macrophages (BMDMs), ABCF1 was manipulated through either overexpression (Over Exp.) or treatment with specific siRNA. These interventions were carried out both in the presence and absence of lipopolysaccharide (LPS) stimulation (at a concentration of 100 ng/ml for 24 hours). The resulting heat map illustrates the fold change in various cytokines and chemokines detected in the cell culture supernatants. B) Similarly, in BMDMs, ABCF1 was modulated using Abcf1-specific siRNA, and these cells were exposed to LPS treatment. The heat map presented here showcases the fold change in various Mitogen-Activated Protein Kinase (MAPK) and Interferon-Stimulated Gene (ISG)-specific phosphoproteins and transcription factors detected in whole-cell lysates (WCL).
    Figure Legend Snippet: A) In bone marrow-derived macrophages (BMDMs), ABCF1 was manipulated through either overexpression (Over Exp.) or treatment with specific siRNA. These interventions were carried out both in the presence and absence of lipopolysaccharide (LPS) stimulation (at a concentration of 100 ng/ml for 24 hours). The resulting heat map illustrates the fold change in various cytokines and chemokines detected in the cell culture supernatants. B) Similarly, in BMDMs, ABCF1 was modulated using Abcf1-specific siRNA, and these cells were exposed to LPS treatment. The heat map presented here showcases the fold change in various Mitogen-Activated Protein Kinase (MAPK) and Interferon-Stimulated Gene (ISG)-specific phosphoproteins and transcription factors detected in whole-cell lysates (WCL).

    Techniques Used: Derivative Assay, Over Expression, Concentration Assay, Cell Culture

    A) The absorbance value associated with phagocytosis was assessed in bone marrow-derived macrophages (BMDMs) that were subjected to ABCF1-specific siRNA treatment. These BMDMs were incubated with fluorescently labeled IgG-coated latex beads for a duration of 2 hours, both in the presence and absence of lipopolysaccharide (LPS). Additionally, ABCF1-specific siRNA-treated BMDMs were incubated with fluorescently labeled E. coli for 2 hours. The results provide insights into the impact of ABCF1 modulation on phagocytosis; B ) The levels of CD16 and CD64 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. C ) The levels of CD32 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. This analysis sheds light on how ABCF1 manipulation influences CD32 expression; C ) In BMDMs, ABCF1 was either overexpressed or not, and these cells were exposed to LPS. Whole-cell lysates (WCL) were immunoprecipitated with an anti-CD32 antibody. The immunoprecipitates were subsequently subjected to immunoblotting using anti-CD32 and anti-phosphotyrosine antibodies. This experimental approach allows the investigation of ABCF1’s role in CD32-associated signaling events; D ) The heat map represents the fold change in phosphorylation levels of Src Family Kinases (SFKs) detected in whole-cell lysates (WCL) from ABCF1-specific siRNA-treated and LPS-stimulated BMDMs. This analysis provides insights into how ABCF1 modulation affects SFK phosphorylation levels in this context.
    Figure Legend Snippet: A) The absorbance value associated with phagocytosis was assessed in bone marrow-derived macrophages (BMDMs) that were subjected to ABCF1-specific siRNA treatment. These BMDMs were incubated with fluorescently labeled IgG-coated latex beads for a duration of 2 hours, both in the presence and absence of lipopolysaccharide (LPS). Additionally, ABCF1-specific siRNA-treated BMDMs were incubated with fluorescently labeled E. coli for 2 hours. The results provide insights into the impact of ABCF1 modulation on phagocytosis; B ) The levels of CD16 and CD64 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. C ) The levels of CD32 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. This analysis sheds light on how ABCF1 manipulation influences CD32 expression; C ) In BMDMs, ABCF1 was either overexpressed or not, and these cells were exposed to LPS. Whole-cell lysates (WCL) were immunoprecipitated with an anti-CD32 antibody. The immunoprecipitates were subsequently subjected to immunoblotting using anti-CD32 and anti-phosphotyrosine antibodies. This experimental approach allows the investigation of ABCF1’s role in CD32-associated signaling events; D ) The heat map represents the fold change in phosphorylation levels of Src Family Kinases (SFKs) detected in whole-cell lysates (WCL) from ABCF1-specific siRNA-treated and LPS-stimulated BMDMs. This analysis provides insights into how ABCF1 modulation affects SFK phosphorylation levels in this context.

    Techniques Used: Derivative Assay, Incubation, Labeling, Flow Cytometry, Fluorescence, Standard Deviation, Expressing, Immunoprecipitation, Western Blot, Phospho-proteomics

    ABCF1 plays a crucial role in orchestrating the intricate process of phagocytosis, particularly in the engulfment of E. coli particles mediated by Fcγ receptors (FcγRs). The sequence of events involving ABCF1 in this process unfolds as follows: 1 ) ABCF1 is essential for the phosphorylation of Immunoreceptor Tyrosine-Based Activation Motifs (ITAMs) by Src Family Kinases (SFKs) such as SRC, LYN, or FGR. This initial phosphorylation event is the trigger for FcγR-mediated phagocytosis of E. coli particles; 2 ) Following ITAM phosphorylation, ABCF1 takes on the role of targeting Spleen Tyrosine Kinase (SYK) for K63-linked polyubiquitination. This ubiquitination event is crucial for subsequent signaling events; 3 ) SYK, once polyubiquitinated, undergoes phosphorylation mediated by SFKs, such as FYN and other members of the SFK family. These phosphorylation events initiate downstream signaling pathways; 4 ) Phosphorylated SYK sets off a cascade of events, including the phosphorylation of Phospholipase C Gamma 2 (PLCγ2), FYN, and other SFKs. These signaling molecules play critical roles in actin polymerization and the formation of the phagocytic cup, a structural prerequisite for successful phagocytosis; 5 ) The downstream consequences of this signaling cascade include elevated phosphorylation levels of Signal Transducer and Activator of Transcription (STAT) proteins, particularly STAT 2, 3, and 6. These phosphorylated STAT proteins contribute to an increase in the production of anti-inflammatory cytokines. In summary, ABCF1’s involvement in phagocytosis of E. coli particles revolves around its role in initiating and coordinating a series of signaling events that ultimately lead to successful engulfment and the production of anti-inflammatory cytokines.
    Figure Legend Snippet: ABCF1 plays a crucial role in orchestrating the intricate process of phagocytosis, particularly in the engulfment of E. coli particles mediated by Fcγ receptors (FcγRs). The sequence of events involving ABCF1 in this process unfolds as follows: 1 ) ABCF1 is essential for the phosphorylation of Immunoreceptor Tyrosine-Based Activation Motifs (ITAMs) by Src Family Kinases (SFKs) such as SRC, LYN, or FGR. This initial phosphorylation event is the trigger for FcγR-mediated phagocytosis of E. coli particles; 2 ) Following ITAM phosphorylation, ABCF1 takes on the role of targeting Spleen Tyrosine Kinase (SYK) for K63-linked polyubiquitination. This ubiquitination event is crucial for subsequent signaling events; 3 ) SYK, once polyubiquitinated, undergoes phosphorylation mediated by SFKs, such as FYN and other members of the SFK family. These phosphorylation events initiate downstream signaling pathways; 4 ) Phosphorylated SYK sets off a cascade of events, including the phosphorylation of Phospholipase C Gamma 2 (PLCγ2), FYN, and other SFKs. These signaling molecules play critical roles in actin polymerization and the formation of the phagocytic cup, a structural prerequisite for successful phagocytosis; 5 ) The downstream consequences of this signaling cascade include elevated phosphorylation levels of Signal Transducer and Activator of Transcription (STAT) proteins, particularly STAT 2, 3, and 6. These phosphorylated STAT proteins contribute to an increase in the production of anti-inflammatory cytokines. In summary, ABCF1’s involvement in phagocytosis of E. coli particles revolves around its role in initiating and coordinating a series of signaling events that ultimately lead to successful engulfment and the production of anti-inflammatory cytokines.

    Techniques Used: Sequencing, Phospho-proteomics, Activation Assay, Ubiquitin Proteomics, Protein-Protein interactions



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    abcf1 gene knockdown  (Santa Cruz Biotechnology)
    92
    Santa Cruz Biotechnology abcf1 gene knockdown
    A) In bone marrow-derived macrophages (BMDMs), <t>ABCF1</t> was manipulated through either overexpression (Over Exp.) or treatment with specific siRNA. These interventions were carried out both in the presence and absence of lipopolysaccharide (LPS) stimulation (at a concentration of 100 ng/ml for 24 hours). The resulting heat map illustrates the fold change in various cytokines and chemokines detected in the cell culture supernatants. B) Similarly, in BMDMs, ABCF1 was modulated using Abcf1-specific siRNA, and these cells were exposed to LPS treatment. The heat map presented here showcases the fold change in various Mitogen-Activated Protein Kinase (MAPK) and Interferon-Stimulated Gene (ISG)-specific phosphoproteins and transcription factors detected in whole-cell lysates (WCL).
    Abcf1 Gene Knockdown, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abcf1 gene knockdown/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    abcf1 gene knockdown - by Bioz Stars, 2026-03
    92/100 stars
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    A) In bone marrow-derived macrophages (BMDMs), ABCF1 was manipulated through either overexpression (Over Exp.) or treatment with specific siRNA. These interventions were carried out both in the presence and absence of lipopolysaccharide (LPS) stimulation (at a concentration of 100 ng/ml for 24 hours). The resulting heat map illustrates the fold change in various cytokines and chemokines detected in the cell culture supernatants. B) Similarly, in BMDMs, ABCF1 was modulated using Abcf1-specific siRNA, and these cells were exposed to LPS treatment. The heat map presented here showcases the fold change in various Mitogen-Activated Protein Kinase (MAPK) and Interferon-Stimulated Gene (ISG)-specific phosphoproteins and transcription factors detected in whole-cell lysates (WCL).

    Journal: bioRxiv

    Article Title: Phagocytosis in macrophages is regulated by the ATP-binding cassette family gene ABCF1

    doi: 10.1101/2023.09.05.556419

    Figure Lengend Snippet: A) In bone marrow-derived macrophages (BMDMs), ABCF1 was manipulated through either overexpression (Over Exp.) or treatment with specific siRNA. These interventions were carried out both in the presence and absence of lipopolysaccharide (LPS) stimulation (at a concentration of 100 ng/ml for 24 hours). The resulting heat map illustrates the fold change in various cytokines and chemokines detected in the cell culture supernatants. B) Similarly, in BMDMs, ABCF1 was modulated using Abcf1-specific siRNA, and these cells were exposed to LPS treatment. The heat map presented here showcases the fold change in various Mitogen-Activated Protein Kinase (MAPK) and Interferon-Stimulated Gene (ISG)-specific phosphoproteins and transcription factors detected in whole-cell lysates (WCL).

    Article Snippet: Specifically, ABCF1 gene knockdown was accomplished using Mouse Abcf1 siRNA from Santa Cruz Biotechnology (catalog number sc-140760), resulting in a consistent reduction of 79-81% compared to control samples treated with scrambled siRNA.

    Techniques: Derivative Assay, Over Expression, Concentration Assay, Cell Culture

    A) The absorbance value associated with phagocytosis was assessed in bone marrow-derived macrophages (BMDMs) that were subjected to ABCF1-specific siRNA treatment. These BMDMs were incubated with fluorescently labeled IgG-coated latex beads for a duration of 2 hours, both in the presence and absence of lipopolysaccharide (LPS). Additionally, ABCF1-specific siRNA-treated BMDMs were incubated with fluorescently labeled E. coli for 2 hours. The results provide insights into the impact of ABCF1 modulation on phagocytosis; B ) The levels of CD16 and CD64 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. C ) The levels of CD32 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. This analysis sheds light on how ABCF1 manipulation influences CD32 expression; C ) In BMDMs, ABCF1 was either overexpressed or not, and these cells were exposed to LPS. Whole-cell lysates (WCL) were immunoprecipitated with an anti-CD32 antibody. The immunoprecipitates were subsequently subjected to immunoblotting using anti-CD32 and anti-phosphotyrosine antibodies. This experimental approach allows the investigation of ABCF1’s role in CD32-associated signaling events; D ) The heat map represents the fold change in phosphorylation levels of Src Family Kinases (SFKs) detected in whole-cell lysates (WCL) from ABCF1-specific siRNA-treated and LPS-stimulated BMDMs. This analysis provides insights into how ABCF1 modulation affects SFK phosphorylation levels in this context.

    Journal: bioRxiv

    Article Title: Phagocytosis in macrophages is regulated by the ATP-binding cassette family gene ABCF1

    doi: 10.1101/2023.09.05.556419

    Figure Lengend Snippet: A) The absorbance value associated with phagocytosis was assessed in bone marrow-derived macrophages (BMDMs) that were subjected to ABCF1-specific siRNA treatment. These BMDMs were incubated with fluorescently labeled IgG-coated latex beads for a duration of 2 hours, both in the presence and absence of lipopolysaccharide (LPS). Additionally, ABCF1-specific siRNA-treated BMDMs were incubated with fluorescently labeled E. coli for 2 hours. The results provide insights into the impact of ABCF1 modulation on phagocytosis; B ) The levels of CD16 and CD64 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. C ) The levels of CD32 were analyzed using flow cytometry in treated BMDMs. The bar graph depicts the change in mean fluorescence intensity (MFI), and error bars indicate the standard deviation. This analysis sheds light on how ABCF1 manipulation influences CD32 expression; C ) In BMDMs, ABCF1 was either overexpressed or not, and these cells were exposed to LPS. Whole-cell lysates (WCL) were immunoprecipitated with an anti-CD32 antibody. The immunoprecipitates were subsequently subjected to immunoblotting using anti-CD32 and anti-phosphotyrosine antibodies. This experimental approach allows the investigation of ABCF1’s role in CD32-associated signaling events; D ) The heat map represents the fold change in phosphorylation levels of Src Family Kinases (SFKs) detected in whole-cell lysates (WCL) from ABCF1-specific siRNA-treated and LPS-stimulated BMDMs. This analysis provides insights into how ABCF1 modulation affects SFK phosphorylation levels in this context.

    Article Snippet: Specifically, ABCF1 gene knockdown was accomplished using Mouse Abcf1 siRNA from Santa Cruz Biotechnology (catalog number sc-140760), resulting in a consistent reduction of 79-81% compared to control samples treated with scrambled siRNA.

    Techniques: Derivative Assay, Incubation, Labeling, Flow Cytometry, Fluorescence, Standard Deviation, Expressing, Immunoprecipitation, Western Blot, Phospho-proteomics

    ABCF1 plays a crucial role in orchestrating the intricate process of phagocytosis, particularly in the engulfment of E. coli particles mediated by Fcγ receptors (FcγRs). The sequence of events involving ABCF1 in this process unfolds as follows: 1 ) ABCF1 is essential for the phosphorylation of Immunoreceptor Tyrosine-Based Activation Motifs (ITAMs) by Src Family Kinases (SFKs) such as SRC, LYN, or FGR. This initial phosphorylation event is the trigger for FcγR-mediated phagocytosis of E. coli particles; 2 ) Following ITAM phosphorylation, ABCF1 takes on the role of targeting Spleen Tyrosine Kinase (SYK) for K63-linked polyubiquitination. This ubiquitination event is crucial for subsequent signaling events; 3 ) SYK, once polyubiquitinated, undergoes phosphorylation mediated by SFKs, such as FYN and other members of the SFK family. These phosphorylation events initiate downstream signaling pathways; 4 ) Phosphorylated SYK sets off a cascade of events, including the phosphorylation of Phospholipase C Gamma 2 (PLCγ2), FYN, and other SFKs. These signaling molecules play critical roles in actin polymerization and the formation of the phagocytic cup, a structural prerequisite for successful phagocytosis; 5 ) The downstream consequences of this signaling cascade include elevated phosphorylation levels of Signal Transducer and Activator of Transcription (STAT) proteins, particularly STAT 2, 3, and 6. These phosphorylated STAT proteins contribute to an increase in the production of anti-inflammatory cytokines. In summary, ABCF1’s involvement in phagocytosis of E. coli particles revolves around its role in initiating and coordinating a series of signaling events that ultimately lead to successful engulfment and the production of anti-inflammatory cytokines.

    Journal: bioRxiv

    Article Title: Phagocytosis in macrophages is regulated by the ATP-binding cassette family gene ABCF1

    doi: 10.1101/2023.09.05.556419

    Figure Lengend Snippet: ABCF1 plays a crucial role in orchestrating the intricate process of phagocytosis, particularly in the engulfment of E. coli particles mediated by Fcγ receptors (FcγRs). The sequence of events involving ABCF1 in this process unfolds as follows: 1 ) ABCF1 is essential for the phosphorylation of Immunoreceptor Tyrosine-Based Activation Motifs (ITAMs) by Src Family Kinases (SFKs) such as SRC, LYN, or FGR. This initial phosphorylation event is the trigger for FcγR-mediated phagocytosis of E. coli particles; 2 ) Following ITAM phosphorylation, ABCF1 takes on the role of targeting Spleen Tyrosine Kinase (SYK) for K63-linked polyubiquitination. This ubiquitination event is crucial for subsequent signaling events; 3 ) SYK, once polyubiquitinated, undergoes phosphorylation mediated by SFKs, such as FYN and other members of the SFK family. These phosphorylation events initiate downstream signaling pathways; 4 ) Phosphorylated SYK sets off a cascade of events, including the phosphorylation of Phospholipase C Gamma 2 (PLCγ2), FYN, and other SFKs. These signaling molecules play critical roles in actin polymerization and the formation of the phagocytic cup, a structural prerequisite for successful phagocytosis; 5 ) The downstream consequences of this signaling cascade include elevated phosphorylation levels of Signal Transducer and Activator of Transcription (STAT) proteins, particularly STAT 2, 3, and 6. These phosphorylated STAT proteins contribute to an increase in the production of anti-inflammatory cytokines. In summary, ABCF1’s involvement in phagocytosis of E. coli particles revolves around its role in initiating and coordinating a series of signaling events that ultimately lead to successful engulfment and the production of anti-inflammatory cytokines.

    Article Snippet: Specifically, ABCF1 gene knockdown was accomplished using Mouse Abcf1 siRNA from Santa Cruz Biotechnology (catalog number sc-140760), resulting in a consistent reduction of 79-81% compared to control samples treated with scrambled siRNA.

    Techniques: Sequencing, Phospho-proteomics, Activation Assay, Ubiquitin Proteomics, Protein-Protein interactions